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Assay is a state-of-the-art in vitro assay that does things. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec porttitor, dolor nec congue varius, eros mauris molestie odio, ut dapibus erat lacus eget leo. Vivamus eget eleifend quam, ac finibus dolor. Quisque fringilla lacus metus, sit amet ornare eros euismod quis. Nam facilisis vehicula sem, sed sodales mi efficitur at. Fusce in scelerisque lectus. Donec et elit ac massa facilisis feugiat at ac elit. Donec est tellus, euismod eget tristique quis, gravida a felis.

Two scientists pointing at an immunoflorescence microscopy image of differentiated neural rosettes

Key Features

            • Animal-free teratogenicity testing
            • Highly sensitivity and specific identification of teratogenic compounds
            • Predict in vivo teratogenicity and extrapolate it to humans
            • More information with less compound compared to animal studies
            • Visualization of the key cellular events of early embryonic development
            • Extensively validated NAM for next generation testing

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Highlights of Assay

  • Unique in vitro screening platform for development toxicity identification
  • Differentiation of hiPSCs towards cardiomyocytes, hepatocytes and neural rosettes
  • Morphological and functional profiling of different tissues
  • Biomarker expression analysis as a readout for teratogenicity
  • Excellent predictor for developmental toxicity hazards

Schematic representation of hiPSCs differentiation towards cardiomyocytes, hepatocytes and neural rosettes in the ReproTracker assay

Typical applications of Assay

  • Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec porttitor, dolor nec congue varius, eros mauris molestie odio, ut dapibus erat lacus eget leo.
  • Vivamus eget eleifend quam, ac finibus dolor. Quisque fringilla lacus metus, sit amet ornare eros euismod quis. Nam facilisis vehicula sem, sed sodales mi efficitur at. Fusce in scelerisque lectus. Donec et elit ac massa facilisis feugiat at ac elit.
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Meet the study director for Assay

Our study directors are the experts in the field to whom you can ask any question about our assays. From early screening to regulatory safety assessment, our study directors are able to listen to your questions and think along with you to provide a tailored solution. Here are some typical questions they often receive on Assay in understanding where and how Assay can be utilized in their strategies. Please feel free to ask your questions as well.

Can I meet you to talk about my study design?

Click for the answer


Will you also take me through the data after a project in a TC or meeting?

Click for the answer

Dr. Amer Jamalpoor
Dr. Amer Jamalpoor
Study director for ReproTracker

Amer Jamalpoor obtained his Ph.D. degree in the field of human Toxicology and Pharmacology. His research focused on the development of in vitro screening platforms for predictive toxicology that utilize human stem cells combined with omics-based biomarker identification. Dr. Jamalpoor joined Toxys as a senior scientist, utilizing his expertise in molecular and cellular biology to run and commercialize the ReproTracker assay. Amer is instrumental in developing and extending the applicability domain of ReproTracker.

Can you help with selecting relevant controls for my substances?

Click for the answer

Click here to send your question!

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec porttitor, dolor nec congue varius, eros mauris molestie odio, ut dapibus erat lacus eget leo. Vivamus eget eleifend quam, ac finibus dolor. Quisque fringilla lacus metus, sit amet ornare eros euismod quis. Nam facilisis vehicula sem, sed sodales mi efficitur at. Fusce in scelerisque lectus. Donec et elit ac massa facilisis feugiat at ac elit. Donec est tellus, euismod eget tristique quis, gravida a felis.

ReproTracker experimental design

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Example results

Example results

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec porttitor, dolor nec congue varius, eros mauris molestie odio, ut dapibus erat lacus eget leo. Vivamus eget eleifend quam, ac finibus dolor. Quisque fringilla lacus metus, sit amet ornare eros euismod quis. Nam facilisis vehicula sem, sed sodales mi efficitur at. Fusce in scelerisque lectus. Donec et elit ac massa facilisis feugiat at ac elit. Donec est tellus, euismod eget tristique quis, gravida a felis.

Project report

The project report contains the protocol, technical details, cytotoxicity testing/dose range finding results, biomarker expression with test
compounds, morphological analysis with test compounds, and LOAEL concentrations of the compounds. We are happy to share the dummy report with you. Please click here to request for a dummy report.

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Donec porttitor, dolor nec congue varius, eros mauris molestie odio, ut dapibus erat lacus eget leo. Vivamus eget eleifend quam, ac finibus dolor. Quisque fringilla lacus metus, sit amet ornare eros euismod quis. Nam facilisis vehicula sem, sed sodales mi efficitur at. Fusce in scelerisque lectus. Donec et elit ac massa facilisis feugiat at ac elit. Donec est tellus, euismod eget tristique quis, gravida a felis.

 

 

Meet the study director for Assay

Our study directors are the experts in the field to whom you can ask any question about our assays. From early screening to regulatory safety assessment, our study directors are able to listen to your questions and think along with you to provide a tailored solution. Here are some typical questions they often receive on Assay in understanding where and how Assay can be utilized in their strategies. Please feel free to ask your questions as well.

Can I meet you to talk about my study design?

Click for the answer


Will you also take me through the data after a project in a TC or meeting?

Click for the answer

Dr. Amer Jamalpoor
Dr. Amer Jamalpoor
Study director for ReproTracker

Amer Jamalpoor obtained his Ph.D. degree in the field of human Toxicology and Pharmacology. His research focused on the development of in vitro screening platforms for predictive toxicology that utilize human stem cells combined with omics-based biomarker identification. Dr. Jamalpoor joined Toxys as a senior scientist, utilizing his expertise in molecular and cellular biology to run and commercialize the ReproTracker assay. Amer is instrumental in developing and extending the applicability domain of ReproTracker.

Can you help with selecting relevant controls for my substances?

Click for the answer

Click here to send your question!

What endpoints are covered by the assay?

ReproTracker detects disruption of stem cell differentiation and early embryonic development. The assay combines the functional/morphological changes and expression of the selected biomarkers in three embryonic lineages (endo-, meso-, and ectoderm) to reveal the developmental toxicity of chemicals.

What is a typical application of ReproTracker?

ReproTracker is an in vitro assay to predict in vivo teratogenicity. The assay is typically applied as an early-phase developmental toxicity screening tool, as an alternative to short-term in vivo testing or as a human follow-up test to investigate inter-species differences and extrapolate animal-derived results to humans.

How do you classify compounds as teratogens?

Compounds are classified as in vitro teratogens when the differentiation of hiPS cells into functional heart, liver or neural cell lineages is disrupted. This disruption is defined by reduction in gene expression of selected biomarkers as well as functional and morphological changes that are induced by the test compound.

What type of chemistry has been tested in the assay?

ReproTracker has been validated with a wide range of chemistry including pharmaceuticals, (agro)chemicals, cosmetics ingredients, and food ingredients.

How is the assay validated?

ReproTracker has been extensively validated using various reference compound libraries as suggested by ICH and EURL ECVAM. These libraries exist of well-established teratogenic and non-teratogenic compounds with different mechanism of actions.

What is the sensitivity and specificity of the assay?

Validation has shown that ReproTracker has an 85% accuracy for identification of in vivo teratogenicity, with a sensitivity of 85% and specificity of 84%.

How much compound is needed to perform the assay?

Compound requirement depends strongly on the toxicity and maximum soluble concentration. Typically, 20-100 mg is sufficient for a full test, including a dose finding and two independent biological test repeats.

How long does it take to receive a report?

Depending on the size of the project, it takes approximately 8-10 weeks to complete a full ReproTracker study and deliver a final report.

What type of solvent can be used?

ReproTracker has been validated with DMSO, water, and PBS as solvents.

What is the top-concentration that is tested, and how is this determined?

The top concentration that is applied in ReproTracker is determined by the toxicity of the compound. The dose that induced 20-40% cytotoxicity is selected as top concentration. Alternatively, the maximum soluble concentration is used. In case no toxicity is observed, 1mM is used as top concentration.

How many compound concentrations are tested?

In the standard ReproTracker protocol, 5 concentrations, typically in 2-fold dilution steps, are tested. Customization of the protocol can be discussed with the study director.

Can you share a sample report?

Yes, please contact us for a sample report via info@toxys.com

Meet the study director for Assay

Our study directors are the experts in the field to whom you can ask any question about our assays. From early screening to regulatory safety assessment, our study directors are able to listen to your questions and think along with you to provide a tailored solution. Here are some typical questions they often receive on Assay in understanding where and how Assay can be utilized in their strategies. Please feel free to ask your questions as well.

Can I meet you to talk about my study design?

Click for the answer


Will you also take me through the data after a project in a TC or meeting?

Click for the answer

Dr. Amer Jamalpoor
Dr. Amer Jamalpoor
Study director for ReproTracker

Amer Jamalpoor obtained his Ph.D. degree in the field of human Toxicology and Pharmacology. His research focused on the development of in vitro screening platforms for predictive toxicology that utilize human stem cells combined with omics-based biomarker identification. Dr. Jamalpoor joined Toxys as a senior scientist, utilizing his expertise in molecular and cellular biology to run and commercialize the ReproTracker assay. Amer is instrumental in developing and extending the applicability domain of ReproTracker.

Can you help with selecting relevant controls for my substances?

Click for the answer

Click here to send your question!

Service

We perform ReproTracker as a service for our clients. You can send your compounds and receive a full report within 8-10 weeks. It is also possible to request a ReproTracker test via scientist.com. Are you interested in receiving a quote or do you have any questions? Please reach out!

Practical information

Compound requirement

  • 20-100 mg test material requested
  • Top concentration tested in ReproTracker is 1 mM or 1 mg/ml

Turn around time

  • 8-10 weeks

Throughput

  • Testing up to 20 compounds per week

Type of solvents compatible with the assay

  • DMSO
  • PBS
  • Water

A standard ReproTracker test includes

  • Extensive dose range finding
  • Two biological repeats
  • Compound tested at five concentrations
  • Positive and negative control compounds included

Next steps after sharing your interest in Assay

Meet the study director for Assay

Our study directors are the experts in the field to whom you can ask any question about our assays. From early screening to regulatory safety assessment, our study directors are able to listen to your questions and think along with you to provide a tailored solution. Here are some typical questions they often receive on Assay in understanding where and how Assay can be utilised in their strategies. Please feel free to ask your questions as well.

Can I meet you to talk about my study design?

Click for the answer


Will you also take me through the data after a project in a TC or meeting?

Click for the answer

Dr. Amer Jamalpoor
Dr. Amer Jamalpoor
Study director for ReproTracker

Amer Jamalpoor obtained his Ph.D. degree in the field of human Toxicology and Pharmacology. His research focused on the development of in vitro screening platforms for predictive toxicology that utilize human stem cells combined with omics-based biomarker identification. Dr. Jamalpoor joined Toxys as a senior scientist, utilizing his expertise in molecular and cellular biology to run and commercialize the ReproTracker assay. Amer is instrumental in developing and extending the applicability domain of ReproTracker.

Can you help with selecting relevant controls for my substances?

Click for the answer

Click here to send your question!

Ask a question or get a quote