Toxys has developed ToxTracker and ToxTracker ACE, unique flow cytometry-based assays that identify various genotoxic and non-genotoxic properties of chemicals and pharmaceuticals that are associated with increased cancer risk. To accommodate in-house use of the assays Toxys now also offers them as a kit. The lab interested in using the assay will first be trained and validated as ToxTracker certified laboratory. After certification the kits can be purchased as required (each kit is sufficient to test 8 compounds +/- S9 in triplo) to allow flexible use and extensive insight into (geno)toxicity for your compounds.
Advantages
- Unique flow cytometry-based assays
- Mechanistic insight
- Identify various genotoxic and non-genotoxic properties in one assay
- Easy step-in for in-house use
- Flexibility
Available Kits
We offer kits for both ToxTracker and ToxTracker ACE. ToxTracker is a unique genotoxicity assay that combines multiple biomarkers to get mechanistic insight into the of action of genotoxic compounds. The assay not only includes markers for DNA damage as predictors for the standard genotoxicity assays, but also includes markers for non-genotoxic MOA, including oxidative stress, protein misfolding and general cellular stress. All these types of cellular damage have been associated with increased cancer risk. ToxTracker ACE (Aneugen Clastogen Evaluation) is an extended version of the ToxTracker assay and includes direct discrimination between an aneugenic and a clastogenic mode-of-action of genotoxic compounds. ToxTracker ACE combines the power of 6 fluorescent reporter cell lines for detection of DNA damage, oxidative stress and protein damage with cell cycle analyses and detection of polyploidy. This unique combination gives unparalleled mechanistic insight into the hazardous properties of chemicals and pharmaceuticals in a single assay. Below you will find an overview of the contents and order numbers for the various kits:
ToxTracker Starter Package
TTSK-101-01 for EU/ TTSK-101-02 for US
Complete package for in-house installation, training and validation
- 6 ToxTracker reporter cell lines
- Wilde type stem cells
- Cell culture reagents
- S9 metabolising system
- Positive control compounds
- 8 validation compounds
- On-site installation support
- Technical assistance during validation
Key features:
- ToxTracker Lab certification
- Sufficient for full analysis of an additional 2 compounds
ToxTracker Kit
TTP-101-01
Kit for full analysis of 8 compounds
- 6 ToxTracker reporter cell lines
- Wilde type stem cells
- Cell culture reagents
- S9 metabolising system
- Positive control compounds
Key features:
- Rapid and reliable genotoxicity assessment
- Discrimination between direct and indirect genotoxicity
- Detection of oxidative stress and protein damage
ToxTracker ACE Kit
TTP-102-01
Kit for full analysis of 8 compounds
- 6 ToxTracker reporter cell lines
- Wilde type stem cells
- Cell culture reagents
- Reagents for assessment of cell cycle and aneuploidy
- S9 metabolising system
- Positive control compounds
Key features:
- Rapid and reliable genotoxicity assessment
- Discrimination between aneugens and clastogens
- Discrimination between direct and indirect genotoxicity
- Detection of oxidative stress and protein damage
Transferability of the assay
Currently a ringtrial for ToxTracker is ongoing, for this the assay has been transferred to 8 laboratories for independant testing. The results from this ring trial form the basis for an OECD application for ToxTracker. The protocols for the assays have been established for the ring trial, and so far the assay has been succesfully transferred to the participants. In a smaller validation study we have run previously we also installed the assay in 2 laboratories, the results demonstrating the transferability of the assay are available in this publication presented at the SOT 2018.
Damian McHugh, manager cell & Genetic Toxicology at Philip Morris Products:
“As a modern safety laboratory requiring mechanistic insights into mammalian toxicology, we adopted ToxTracker and installed the assay in-house. The expertise, training and support provided by Toxys for the technology transfer were excellent resulting in a seamless set-up of the assay in our facilities. In addition, we have had terrific reactivity from Giel and his team with our questions..
Training and certification
We are very proud on the consistency and quality of the ToxTracker assays. To guarantee the same for our customers using the assay in-house we only offer the Kits to labs who have had the training and have been certified (certification is included in the ToxTracker starter package). The training entails a 3-5 day on-site visit from one of our scientist who will perform a hands-on training. You will gain experience with culturing the cells and performing the analysis. After the training you will be able to perform the assay yourself. All relevant documents, such as calculation sheets and protocols will be shared before the training. By testing a number of reference compounds we will assess whether results are in line with our expectations and quality criteria. When this is finalised you will get a ToxTracker certification allowing you to order further ToxTracker Kits.
Technical Requirements
To run ToxTracker in-house you will need a cell culturing laboratory and a flow cytometer.
Flow cytometer requirements to run ToxTracker assay
- Suitable for 96-well plates
- (Orbital) shaker or needle with mixing capacity for sample uptake
- Laser for GFP (488 nm)
Flow cytometer requirements to run ToxTracker ACE assay
- Suitable for 96-well plates
- (Orbital) shaker or needle with mixing capacity for sample uptake
- Laser for GFP (488 nm)
- Laser for Hoechst (405 nm) or laser for Orange Dye (488 nm or 532 nm)
- Ability to measure peak area, height and width in flow cytometer
- FACS data analysis software for cell cycle and polyploidy analysis
How to order?
If you are interested in using one of the Kits in-house or need advice on which kit would be most suitable for your project please contact us via the button below of by sending an email to orders@toxys.com.
