We offer an unique panel of cell lines with deficiencies in the major DNA repair systems which can be employed to determine the cellular pathways that protect cells against the toxic properties of chemicals.
Tools available for:
- Unique collection of mammalian DNA repair mutant cell lines
- Extensive expertise in the field of DNA repair and mutagenesis
- Assessment of cytotoxicity and genotoxicity in absence of DNA repair
Importance of DNA repair systems
Organisms possess various cellular defense mechanisms that provide protection against the detrimental consequences of chemical exposure. DNA repair systems such as base excision repair (BER) and nucleotide excision repair (NER) systems efficiently remove small base pair damages or larger bulky adducts from the DNA before they can become mutagenic. Double-strand DNA breaks that may arise directly from exposure or as a result from interference of DNA lesions with DNA replication and transcription, are generally repaired by non-homologous end joining (NHEJ) or homologous recombination (HR). Unrepaired DNA lesions may result in the formation of genetic alterations which can drive carcinogenesis.
Unique panel of mammalian cell lines with deficiencies in the major DNA repair systems
Toxys offers a unique collection of mammalian cell lines to determine the impact of the various DNA repair system on the cytotoxicity and genotoxicity of chemicals. This panel of cell lines has deficiencies in the major DNA repair systems can be employed to determine the cellular pathways that protect cells against the toxic properties of chemicals. The major DNA damage repair system that have been described today are (I) Base excision repair (BER) for repair of small non-helix distorting base damages, (II) Nucleotide excision repair (NER) for repair of bulky helix-distorting DNA lesions and intra-strand cross links and (III) DNA double strand break repair by either non-homologs end joining (NHEJ) or homologous recombination (HR). Repair of DNA inter-strand cross links can involve specific factors but often relies on a combination of repair enzymes that are involved in the DNA repair systems indicated above. Nucleotide excision repair can be further dissected into global genome NER and transcription-coupled NER which are different in the mechanism of DNA lesion recognition.
Our unique panel consist of 11 cell lines to assess effects on:
- Base excision repair
- Nucleotide excision repair
- Double strand break repair
- DNA mismatch repair
- Translesion synthesis
Application and Access
The cell lines can be used for various application; to understand more about the mechanism of toxicity caused by compounds, as a tool for drug discovery to study how DNA repair is affected and as a tool to stratify the best application for a compounds that is targeted towards DNA repair. Access to this panel is offered in fee for service projects