Hprt mutation assays

DNA damage can lead to the induction of gene mutations. Mutagenesis is the driving force behind the development of various genetic diseases including cancer. Toxys has a longstanding experience in various mammalian gene mutation assays.

Petri dishes used in an HPRT mutation assay. Blue dots are visible on the plates, likely representing colonies of cells with mutations in the hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene.

Key Characteristics

  • Mammalian gene mutation assay
  • Quantitative detection of gene mutations
  • Unique combination with ToxTracker

The mouse ES cells that are applied in the ToxTracker assay have been extensively used to study mutagenesis. The hprt, aprt and tk genes are typically used to study DNA damage-induced mutations. These genes allow efficient selection and highly quantitative identification of mutants. Detailed generation of mutation spectra by DNA sequencing can be extremely valuable to assess the nature of DNA lesions that underly the mutations. We can extend the ToxTracker assay that allows the detection of promutagenic DNA lesions with the quantitative assessment of mutation induction following chemical exposure.

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