The ToxTracker assay is a state-of-the-art stem cell-based reporter assay that provides mechanistic insight into genotoxic properties of compounds. ToxTracker ACE (Aneugen Clastogen Evaluation) is an extended version of the ToxTracker assay and includes direct discrimination between an aneugenic and a clastogenic mode-of-action of genotoxic compounds. ToxTracker ACE combines the power of 6 fluorescent reporter cell lines for detection of DNA damage, oxidative stress and protein damage with cell cycle analyses and detection of polyploidy. This unique combination gives unparalleled mechanistic insight into the hazardous properties of chemicals and pharmaceuticals in a single assay. The information generated is particularly useful in Mode-of-Action (MoA) and Adverse-Outcome Pathway (AOP) approaches.
- mechanistic insight into (geno)toxicity
- unsurpassed sensitivity and specificity of 95%
- excellent predictor for regulatory in vitro and in vivo assays
- low amount of compound required
- rapid and cost effective
- metabolic activation using S9 liver extract
ToxTracker ACE is an extended version of the ToxTracker assay and includes cell cycle analyses and polyploidy detection to allow direct discrimination between an aneugenic and clastogenic mode-of-action. ToxTracker ACE (Aneugen Clastogen Evaluation) combines the 6 fluorescent reporter cell lines for detection of DNA damage, oxidative stress and protein damage with a DNA staining for cell cycle and chromosome analysis. The combination of differential activation of these endpoints is a unique and very powerful means to separate clastogens from aneugens, as well as to identify potential tubulin poisons and cell cycle kinase inhibitors.
Unique combination of endpoints
Clastogen vs. Aneugens example
We compared cisplatin (DNA cross linker), taxol (tubulin poison) and AMG900 (kinase inhibitor).
- Activation of both genotoxicity reporters in ToxTracker
- Cell cycle block in S phase
- No polyploidy
- Selective activation of the Rtkn genotoxicity reporter
- Cell cycle block in G2/M phase
- Optional: perform TubulinTracker
- No activation of the ToxTracker genotoxicity reporters
- Cell cycle block in G2/M phase
- Optional: PhosoH3 staining for Aurora kinase B inhibition
From sample to report
This visualisation illustrates the sequence of events between receiving your test compound, performing the ToxTracker ACE and providing the final results.
We can perform ToxTracker ACE for you at our state-of-the-art laboratory. You can then send your compounds and receive a full report, in most cases within 2 weeks. Most of our customers ask us to perform the assay as a fee-for-service project as we have all tools and equipment in house and are experienced in the data interpretation. We work together on this basis with various of the top 10 pharma, chemical and cosmetics companies
Local lab installation
The assay can also be installed in your laboratory under license. We provide full support for integration of ToxTracker ACE in your toxicology laboratory. Please contact us via the contact form below or via email: email@example.com
ToxTracker ACE in Japan via Eolas Biosciences
Toxys has partnered with Eolas Biosciences to include ToxTracker ACE in their portfolio of genetic toxicology services for the Japanese Market. Visit this page to learn more.
ToxTracker ACE via research marketplaces Science Exchange and Scientist.com
Toxys has made its ToxTracker ACE assay available through the research service marketplaces Science Exchange and Scientist.com. These platforms are used worldwide by large and small companies to source their testing services. Organisations listed as buyers can now make use of the standardised legal agreements of these platforms and efficiently order ToxTracker services.
These are some of the typical application of ToxTracker ACE:
- Genotoxicity testing of pharmaceutical compounds
- Identification of aneugens
- Genotoxicity testing of kinase inhibitors