Reporter cell systems can be used to get an easy read-out of gene activation (transcription). By adding a GFP reporter (or other color reporter) to the gene of interest activation of this gene can be monitored by measuring GFP induction, for example by flow-cytometry or imaging.
Key Expertise
- BAC systmes
- CRISPR methods
- cell cloning
- generation of functional assays
Extensive expertise in creating reporter cell systems
Reporter cell systems can be used to get an easy read-out of gene activation (transcription). By adding a GFP reporter to the gene of interest activation of this gene can be monitored by measuring GFP induction, for example by flow-cytometry or imaging.
In the creation of ToxTracker we have used an bacterial artificial chromosome to add the GFP markers, as described in the articles. Within Toxys we have extensive experience in creating reporter assays in using:
- bacterial artificial chromosome
- using GFP or other markers like luminescence or m-cherry
- CRISP
Please feel free to reach out when you are interested to learn more about these reporter systems or if we can help you create a reporter assay.
