These are some examples of how ToxTracker has been applied. If you are interested in learning more about ToxTracker applications or how the assay fits within screening strategies or if you would like to discuss you specific questions please contact us via: firstname.lastname@example.org.
Oxidative stress leading to genotoxicity
High levels of oxidative stress can indirectly lead to genotoxic effects. The AOP has been well established. Because genotoxicity is often observed only at high levels of oxidative stress, there might be a threshold for a safe exposure level. In our ToxTracker AO protocol, we test compounds in the presence of different ROS scavengers and determine whether genotoxicity is caused indirectly by high levels of oxidative stress or rather by direct binding of the compound to the DNA. In this poster, you can find an example of the application of ToxTracker AO for the mode of action assessment of 10 cosmetics compounds.
Equivocal results in regulatory genotoxicity assays
In this particular case, a client obtained equivocal results in the standard battery of in vitro genotoxicity assays, and they were looking for a way to understand the profile of the compound and that could also help justify the classification of the compound. They have tested the compound in ToxTracker which identified the compound as non-genotoxic, but did induce oxidative stress. The customer used this information together with other data in a weight-of-evidence approach to justify that the compound should be classified as not mutagenic. Please read more about this in this case study.
ToxTracker is validated extensively and is currently in an international OECD validation study. The results from ToxTracker have been submitted in various regulatory files. The assay has been applied for testing various chemical modalities including:
- Complex mixtures
- Nano materials