Posters

ToxTracker ACE and TubulinTracker provide mechanistic insight into the mode of action of aneugenic substances

2021 — Understanding the mode of action (MOA) and the classification of clastogens and aneugens is important for regulatory considerations. ToxTracker ACE is a mammalian stem cell-based reporter assay that detects the activation of specific cellular signalling pathways upon exposure to compounds to provide insight into the MOA.

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2021 — Understanding the mode of action (MOA) and the classification of clastogens and aneugens is important for regulatory considerations. ToxTracker ACE is a mammalian stem cell-based reporter assay that detects the activation of specific cellular signalling pathways upon exposure to compounds to provide insight...

ReproTracker: a Human Stem Cell-Based Biomarker Assay for in vitro Assessment of Developmental Toxicity

2021 — Developmental toxicological screening is a crucial part of chemical safety assessment. Alternative in vitro approaches have been introduced to improve the identification of developmental toxicants.

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2021 — Developmental toxicological screening is a crucial part of chemical safety assessment. Alternative in vitro approaches have been introduced to improve the identification of developmental toxicants....

TubulinTracker, a Novel In Vitro Reporter Assay to Study Intracellular Microtubule Dynamics and Cell Cycle Progression

2021 — Aneuploidy, the presence of an abnormal number of chromosomes, can be caused by any process that interferes with chromosome segregation during mitosis, including microtubule disruption or inhibition of the Aurora A/B/C cell cycle kinases.

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2021 — Aneuploidy, the presence of an abnormal number of chromosomes, can be caused by any process that interferes with chromosome segregation during mitosis, including microtubule disruption or inhibition of the Aurora A/B/C cell cycle kinases....

Validation of the ToxProfiler reporter assay for toxicological profiling and determination of the underlying mode of action

2021 — Classical in vitro chemical safety assessment often relies on simple cytotoxicity endpoint measurements. However, the underlying mechanisms are usually poorly understood from these general assays. In order to gain further understanding of cell stress mechanisms we have developed the ToxProfiler assay, a unique new approach method (NAM).

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2021 — Classical in vitro chemical safety assessment often relies on simple cytotoxicity endpoint measurements. However, the underlying mechanisms are usually poorly understood from these general assays. In order to gain further understanding of cell stress mechanisms we have developed the ToxProfiler assay, a unique new...

Evaluation of the ToxTracker® assay for DNA damage and pro-oxidative MoA using 10 coded chemicals

2019 — Unilever’s Safety & Environmental Assurance Centre (SEAC) constantly seeks to bring new scientific solutions when making safety and environmental risk and impact assessments of Unilever products. Novel mechanistic information can be used in the context of making robust safety decisions about the safe use of specific ingredients in consumer products.

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2019 — Unilever’s Safety & Environmental Assurance Centre (SEAC) constantly seeks to bring new scientific solutions when making safety and environmental risk and impact assessments of Unilever products. Novel mechanistic information can be used in the context of making robust safety decisions about the safe...

Combining a genotoxin-specific qPCR method with the ToxTracker to evaluate the genotoxic potential of high priority printed paper and board food contact material substances

2019 — Printed paper and board food contact materials are a potential source of genotoxic food contaminants. Both the ToxTracker and a genotoxin specific qPCR method correctly identified known genotoxic and non-genotoxic food contact material substances.

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2019 — Printed paper and board food contact materials are a potential source of genotoxic food contaminants. Both the ToxTracker and a genotoxin specific qPCR method correctly identified known genotoxic and non-genotoxic food contact material substances....

Development of a stem cell-based reporter assay for DART assessment

2019 — Testing for developmental and reproductive toxicity (DART) is a crucial part of the toxicological risk assessment. To improve the in vitro identification of developmental toxicants, we developed an assay in which we followed differentiation of human induced pluripotent stem cells (hiPSC) towards cardiomyocytes, hepatocytes or neural rosettes by biomarker based qPCR based method.

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2019 — Testing for developmental and reproductive toxicity (DART) is a crucial part of the toxicological risk assessment. To improve the in vitro identification of developmental toxicants, we developed an assay in which we followed differentiation of human induced pluripotent stem cells (hiPSC) towards cardiomyocytes, hepatocytes...

Optimisation of in vitro metabolism using S9 liver extract in ToxTracker

2019 — Progenotoxic agents, such as Aflatoxin B1 and Benzo[a]pyrene, only become DNA reactive after metabolisation by detoxification enzymes. In vivo metabolism takes place in for example the liver, bone marrow or the lungs.

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2019 — Progenotoxic agents, such as Aflatoxin B1 and Benzo[a]pyrene, only become DNA reactive after metabolisation by detoxification enzymes. In vivo metabolism takes place in for example the liver, bone marrow or the lungs....

Extension of the ToxTracker reporter assay for classification of compounds with a clastogenic or aneugenic mode-of-action

2019 — Understanding the mode of action (MOA) and the classification of clastogens and aneugens is important for regulatory considerations. ToxTracker is a mammalian stem cell-based reporter assay that detects the activation of specific cellular signalling pathways upon exposure to compounds to provide insight into the MOA.

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2019 — Understanding the mode of action (MOA) and the classification of clastogens and aneugens is important for regulatory considerations. ToxTracker is a mammalian stem cell-based reporter assay that detects the activation of specific cellular signalling pathways upon exposure to compounds to provide insight into...

Adaptation of the ToxTracker reporter assay for the genetic toxicology assessment of petroleum products

2019 — Poorly refined heavier petroleum substances (PS) have the potential to be mutagenic and / or carcinogenic based on their 3-7 ring poly aromatic hydrocarbon (PAH) content. The petroleum industry applies 2 common methods to screen for these effects, namely the Modified Ames assay and the IP346 test. These tests provide a yes / no answer based on a cut-off value, and are validated against a significant in vivo dataset.

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2019 — Poorly refined heavier petroleum substances (PS) have the potential to be mutagenic and / or carcinogenic based on their 3-7 ring poly aromatic hydrocarbon (PAH) content. The petroleum industry applies 2 common methods to screen for these effects, namely the Modified Ames assay...

The ToxTracker reporter assay detects indirect genotoxicity caused by high levels of oxidative stress

2018 — Current tests for genotoxicity do not specifically investigate oxidative damage, eventhough this process can (indirectly) lead to genotoxicity. ToxTracker is a mammalian stem cell-based reporter assay that detects the activation of specific cellular signaling pathways upon exposure to compounds. The assay can distinguish between DNA damage, protein damage, p53 activation and oxidative damage.

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2018 — Current tests for genotoxicity do not specifically investigate oxidative damage, eventhough this process can (indirectly) lead to genotoxicity. ToxTracker is a mammalian stem cell-based reporter assay that detects the activation of specific cellular signaling pathways upon exposure to compounds. The assay can distinguish...

The ToxTracker assay as a tool for mechanism-based (geno)toxicity screening of nanoparticles

2018 — There is a growing demand for fast and reliable assays to identify nanoparticle-induced toxicity and their underlying mechanisms. The ToxTracker assay consists of six mouse stem cell reporter cell lines that use GFP-tagged biomarker genes for detecting the activation of various cellular signaling pathways relevant for carcinogenesis.

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2018 — There is a growing demand for fast and reliable assays to identify nanoparticle-induced toxicity and their underlying mechanisms. The ToxTracker assay consists of six mouse stem cell reporter cell lines that use GFP-tagged biomarker genes for detecting the activation of various cellular signaling...

Genotoxicity assessment of Antimony compounds using the ToxTracker assay

2018 — The metalloid Antimony (Sb) is commonly used as a compound in flame retardants and as hardener for lead. There is still a lot of discussion on the genotoxicity of antimony compounds. Both trivalent and pentavalent compounds are generally negative in non-mammalian genotoxicity assays, but in mammalian test systems trivalent compounds have generated some positive results, while pentavalent compounds remained negative.

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2018 — The metalloid Antimony (Sb) is commonly used as a compound in flame retardants and as hardener for lead. There is still a lot of discussion on the genotoxicity of antimony compounds. Both trivalent and pentavalent compounds are generally negative in non-mammalian genotoxicity assays,...

The ToxTracker reporter assay detects indirect genotoxicity by high levels of oxidative stress

2018 — ToxTracker is a mammalian stem cell-based reporter assay that detects activation of specific cellular signaling pathways upon exposure to compounds. During the validation of this assay, we found that various compounds that give misleading positive results in the conventional in vitro genotoxicity assays did not activate the DNA damage reporters but did induce high levels of oxidative stress or protein damage in ToxTracker.

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2018 — ToxTracker is a mammalian stem cell-based reporter assay that detects activation of specific cellular signaling pathways upon exposure to compounds. During the validation of this assay, we found that various compounds that give misleading positive results in the conventional in vitro genotoxicity assays...

Validation of the ToxTracker reporter assay for the genetic toxicology assessment of petroleum products

2018 — The ToxTracker reporter cell lines were exposed to 18 DMSO extracts of petroleum substances obtained from various Concawe member companies. The differential induction of the GFP reporters as well as cytotoxicity of the tested substances was determined by flow cytometry.

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2018 — The ToxTracker reporter cell lines were exposed to 18 DMSO extracts of petroleum substances obtained from various Concawe member companies. The differential induction of the GFP reporters as well as cytotoxicity of the tested substances was determined by flow cytometry....

Interlaboratory validation of the ToxTracker genotoxicity reporter assay

2018 — To investigate the reliability and transferability of the ToxTracker assay, we performed a limited interlaboratory validation study. In total 27 compounds (24 test compounds and 3 assay controls) were tested at least three times by two independent laboratories. The within-laboratory reproducibility (WLR) for the six different ToxTracker reporters was assessed per category of reporters (DNA damage, oxidative stress, protein damage) and for all reporters together.

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2018 — To investigate the reliability and transferability of the ToxTracker assay, we performed a limited interlaboratory validation study. In total 27 compounds (24 test compounds and 3 assay controls) were tested at least three times by two independent laboratories. The within-laboratory reproducibility (WLR) for...

Use of multiparametric mode of action approaches for genetic toxicity assessment: ToxTracker reporter cell lines versus gene analysis

2017 — In support of the development of a predictive, animal-free genetic toxicology approach, we investigated several different methodologies with varying degrees of complexity. The predictive capacity of these methods was compared by testing 22 chemicals that represent a mix of DNA reactive chemicals, chemicals with known, mixed or unknown modes of action, and chemicals with well-defined in vivo/vitro genotoxicity outcomes.

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2017 — In support of the development of a predictive, animal-free genetic toxicology approach, we investigated several different methodologies with varying degrees of complexity. The predictive capacity of these methods was compared by testing 22 chemicals that represent a mix of DNA reactive chemicals, chemicals...

Application of the ToxTracker reporter assay in a mode-of-action approach for genetic toxicology assessment

2017 — In an extensive validation study using 300 reference chemicals and >200 proprietary compounds, ToxTracker classified the genotoxic compounds with a sensitivity of 94% and specificity of 95%. By assessing the differential induction of the two DNA damage reporters, ToxTracker was able to discriminate between a mutagenic and clastogenic mechanism of genotoxicity.

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2017 — In an extensive validation study using 300 reference chemicals and >200 proprietary compounds, ToxTracker classified the genotoxic compounds with a sensitivity of 94% and specificity of 95%. By assessing the differential induction of the two DNA damage reporters, ToxTracker was able to discriminate...

Mechanism-based genotoxicity screening of nanomaterials using the ToxTracker panel of reporter cell lines

2017 — ToxTracker is a mammalian stem cell-based reporter assay that detects activation of specific cellular signalling pathways upon exposure to unknown compounds. ToxTracker contains six different GFP-tagged reporters that allow discrimination between induction of DNA damage, oxidative stress and protein damage in a single test.

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2017 — ToxTracker is a mammalian stem cell-based reporter assay that detects activation of specific cellular signalling pathways upon exposure to unknown compounds. ToxTracker contains six different GFP-tagged reporters that allow discrimination between induction of DNA damage, oxidative stress and protein damage in a single...

The ToxTracker reporter system is a mechanistic genotoxicity assay that discriminates clastogenic from aneugenic compounds

2016 — With the increasing production of new chemicals for a wide range of applications in health care, food and cosmetics, there is an urgent need for rapid and reliable toxicity assessment. For reliable prediction of human health hazards, in vitro test systems should ideally not only identify adverse properties of chemicals, but also provide insight into the type of cellular damage inflicted by novel compounds.

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2016 — With the increasing production of new chemicals for a wide range of applications in health care, food and cosmetics, there is an urgent need for rapid and reliable toxicity assessment. For reliable prediction of human health hazards, in vitro test systems should ideally...

Mechanistic toxicity profiling of chemicals and nanomaterials by combining the ToxTracker genotoxicity assay and MiToxView mitochondrial toxicity assay

2015 — The ToxTracker assay uses a panel of mouse embryonic stem (mES) cells that contain different GFP reporter genes for induction of DNA damage, oxidative stress, protein damage and general cytotoxicity. Extensive validation using different reference compound libraries as well as a collection of nanomaterials showed that the integrative approach of the ToxTracker assay is a powerful tool for in vitro carcinogenic hazard identification.

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2015 — The ToxTracker assay uses a panel of mouse embryonic stem (mES) cells that contain different GFP reporter genes for induction of DNA damage, oxidative stress, protein damage and general cytotoxicity. Extensive validation using different reference compound libraries as well as a collection of...