Toxicological Activity Profiling and Potency Ranking of Per‐ and Polyfluoroalkyl Substances (PFAS) Using ToxProfiler
2023 — Per- and polyfluoroalkyl substances (PFAS), also known as “the forever compounds”, have the tendency to accumulate in organisms and their environment. Despite extensive in vitro and in vivo testing of PFAS, there is a remaining concern about the human safety of these chemicals.
Tags: Chemicals,Mechanistic toxicity,ToxProfiler
2023 — Per- and polyfluoroalkyl substances (PFAS), also known as “the forever compounds”, have the tendency to accumulate in organisms and their environment. Despite extensive in vitro and in vivo testing of PFAS, there is a remaining concern about the human safety of these chemicals....
ToxTracker – A Key to Early-Stage Molecule Genetox Testing
2022 — ToxTracker is a unique genotoxicity assay that combines multiple biomarkers through 6 reporter cell lines to evaluate the potential mode of action of genotoxic compounds. The ToxTracker assay is robust and can easily be modified for fit- for-purpose for early-stage screening of the genotoxic potential of molecules.
Tags: Agrochemicals,Genotoxicity,ToxTracker ACE
2022 — ToxTracker is a unique genotoxicity assay that combines multiple biomarkers through 6 reporter cell lines to evaluate the potential mode of action of genotoxic compounds. The ToxTracker assay is robust and can easily be modified for fit- for-purpose for early-stage screening of the...
TubulinTracker, a novel in vitro reporter assay to study intracellular microtubule dynamics
2022 — Aneuploidy, the presence of an abnormal number of chromosomes, can be caused by any process that interferes with chromosome segregation during mitosis, including microtubule disruption or inhibition of cell cycle kinases such as Aurora kinases A/B/C.
Tags: Genotoxicity,TubulinTracker
2022 — Aneuploidy, the presence of an abnormal number of chromosomes, can be caused by any process that interferes with chromosome segregation during mitosis, including microtubule disruption or inhibition of cell cycle kinases such as Aurora kinases A/B/C. ...
Validation of the ToxProfiler reporter assay for toxicological profiling and determination of the underlying mode of action
2022 — Classical in vitro chemical safety assessment often relies on simple and general cytotoxicity endpoint measurements. To improve the in vitro predictions and understand the underlying mechanisms of toxicity, we previously introduced ToxProfiler.
Tags: Mechanistic toxicity,ToxProfiler
2022 — Classical in vitro chemical safety assessment often relies on simple and general cytotoxicity endpoint measurements. To improve the in vitro predictions and understand the underlying mechanisms of toxicity, we previously introduced ToxProfiler. ...
ReproTracker: a human stem cell-based biomarker assay for in vitro assessment of developmental toxicity
2022 — ReproTracker is a human induced pluripotent stem cell (hiPSC)-based biomarker assay, which combines morphological changes and expression of selected biomarkers in different embryonic lineages to reveal developmental toxicity of chemicals.
Tags: Developmental Toxicity,ReproTracker
2022 — ReproTracker is a human induced pluripotent stem cell (hiPSC)-based biomarker assay, which combines morphological changes and expression of selected biomarkers in different embryonic lineages to reveal developmental toxicity of chemicals....
Extension of the ToxTracker reporter assay for mechanistic insight into mode-of-action of genotoxic compounds
2021 —ToxTracker is a mammalian stem cell-based reporter assay that detects the activation of specific cellular signaling pathways upon exposure to compounds. ToxTracker contains six different GFP- tagged reporters that allow the discrimination between the induction of DNA damage, oxidative stress and protein damage in a single test.
Tags: Genotoxicity,ToxTracker ACE,ToxTracker AO
2021 —ToxTracker is a mammalian stem cell-based reporter assay that detects the activation of specific cellular signaling pathways upon exposure to compounds. ToxTracker contains six different GFP- tagged reporters that allow the discrimination between the induction of DNA damage, oxidative stress and protein damage in...
ToxTracker ACE and TubulinTracker provide mechanistic insight into the mode of action of aneugenic substances
2021 — Understanding the mode of action (MOA) and the classification of clastogens and aneugens is important for regulatory considerations. ToxTracker ACE is a mammalian stem cell-based reporter assay that detects the activation of specific cellular signalling pathways upon exposure to compounds to provide insight into the MOA.
Tags: Genotoxicity,ToxTracker ACE,TubulinTracker
2021 — Understanding the mode of action (MOA) and the classification of clastogens and aneugens is important for regulatory considerations. ToxTracker ACE is a mammalian stem cell-based reporter assay that detects the activation of specific cellular signalling pathways upon exposure to compounds to provide insight...
TubulinTracker, a Novel In Vitro Reporter Assay to Study Intracellular Microtubule Dynamics and Cell Cycle Progression
2021 — Aneuploidy, the presence of an abnormal number of chromosomes, can be caused by any process that interferes with chromosome segregation during mitosis, including microtubule disruption or inhibition of the Aurora A/B/C cell cycle kinases.
Tags: Genotoxicity,TubulinTracker
2021 — Aneuploidy, the presence of an abnormal number of chromosomes, can be caused by any process that interferes with chromosome segregation during mitosis, including microtubule disruption or inhibition of the Aurora A/B/C cell cycle kinases....
Evaluation of the ToxTracker® assay for DNA damage and pro-oxidative MoA using 10 coded chemicals
2019 — Unilever’s Safety & Environmental Assurance Centre (SEAC) constantly seeks to bring new scientific solutions when making safety and environmental risk and impact assessments of Unilever products. Novel mechanistic information can be used in the context of making robust safety decisions about the safe use of specific ingredients in consumer products.
Tags: Cosmetics,Genotoxicity,ToxTracker AO
2019 — Unilever’s Safety & Environmental Assurance Centre (SEAC) constantly seeks to bring new scientific solutions when making safety and environmental risk and impact assessments of Unilever products. Novel mechanistic information can be used in the context of making robust safety decisions about the safe...
Combining a genotoxin-specific qPCR method with the ToxTracker to evaluate the genotoxic potential of high priority printed paper and board food contact material substances
2019 — Printed paper and board food contact materials are a potential source of genotoxic food contaminants. Both the ToxTracker and a genotoxin specific qPCR method correctly identified known genotoxic and non-genotoxic food contact material substances.
Tags: Food contact materials,Genotoxicity,ToxTracker
2019 — Printed paper and board food contact materials are a potential source of genotoxic food contaminants. Both the ToxTracker and a genotoxin specific qPCR method correctly identified known genotoxic and non-genotoxic food contact material substances....
Optimisation of in vitro metabolism using S9 liver extract in ToxTracker
2019 — Progenotoxic agents, such as Aflatoxin B1 and Benzo[a]pyrene, only become DNA reactive after metabolisation by detoxification enzymes. In vivo metabolism takes place in for example the liver, bone marrow or the lungs.
Tags: Genotoxicity,ToxTracker
2019 — Progenotoxic agents, such as Aflatoxin B1 and Benzo[a]pyrene, only become DNA reactive after metabolisation by detoxification enzymes. In vivo metabolism takes place in for example the liver, bone marrow or the lungs....
Extension of the ToxTracker reporter assay for classification of compounds with a clastogenic or aneugenic mode-of-action
2019 — Understanding the mode of action (MOA) and the classification of clastogens and aneugens is important for regulatory considerations. ToxTracker is a mammalian stem cell-based reporter assay that detects the activation of specific cellular signalling pathways upon exposure to compounds to provide insight into the MOA.
Tags: Genotoxicity,Pharmaceuticals,ToxTracker ACE
2019 — Understanding the mode of action (MOA) and the classification of clastogens and aneugens is important for regulatory considerations. ToxTracker is a mammalian stem cell-based reporter assay that detects the activation of specific cellular signalling pathways upon exposure to compounds to provide insight into...
Adaptation of the ToxTracker reporter assay for the genetic toxicology assessment of petroleum products
2019 — Poorly refined heavier petroleum substances (PS) have the potential to be mutagenic and / or carcinogenic based on their 3-7 ring poly aromatic hydrocarbon (PAH) content. The petroleum industry applies 2 common methods to screen for these effects, namely the Modified Ames assay and the IP346 test. These tests provide a yes / no answer based on a cut-off value, and are validated against a significant in vivo dataset.
Tags: Genotoxicity,Petroleum products,ToxTracker
2019 — Poorly refined heavier petroleum substances (PS) have the potential to be mutagenic and / or carcinogenic based on their 3-7 ring poly aromatic hydrocarbon (PAH) content. The petroleum industry applies 2 common methods to screen for these effects, namely the Modified Ames assay...
The ToxTracker reporter assay detects indirect genotoxicity caused by high levels of oxidative stress
2018 — Current tests for genotoxicity do not specifically investigate oxidative damage, eventhough this process can (indirectly) lead to genotoxicity. ToxTracker is a mammalian stem cell-based reporter assay that detects the activation of specific cellular signaling pathways upon exposure to compounds. The assay can distinguish between DNA damage, protein damage, p53 activation and oxidative damage.
Tags: Agrochemicals,Genotoxicity,ToxTracker AO
2018 — Current tests for genotoxicity do not specifically investigate oxidative damage, eventhough this process can (indirectly) lead to genotoxicity. ToxTracker is a mammalian stem cell-based reporter assay that detects the activation of specific cellular signaling pathways upon exposure to compounds. The assay can distinguish...
The ToxTracker assay as a tool for mechanism-based (geno)toxicity screening of nanoparticles
2018 — There is a growing demand for fast and reliable assays to identify nanoparticle-induced toxicity and their underlying mechanisms. The ToxTracker assay consists of six mouse stem cell reporter cell lines that use GFP-tagged biomarker genes for detecting the activation of various cellular signaling pathways relevant for carcinogenesis.
Tags: Genotoxicity,Metals,Nanoparticles,ToxTracker
2018 — There is a growing demand for fast and reliable assays to identify nanoparticle-induced toxicity and their underlying mechanisms. The ToxTracker assay consists of six mouse stem cell reporter cell lines that use GFP-tagged biomarker genes for detecting the activation of various cellular signaling...
Genotoxicity assessment of Antimony compounds using the ToxTracker assay
2018 — The metalloid Antimony (Sb) is commonly used as a compound in flame retardants and as hardener for lead. There is still a lot of discussion on the genotoxicity of antimony compounds. Both trivalent and pentavalent compounds are generally negative in non-mammalian genotoxicity assays, but in mammalian test systems trivalent compounds have generated some positive results, while pentavalent compounds remained negative.
Tags: Genotoxicity,Metals,ToxTracker
2018 — The metalloid Antimony (Sb) is commonly used as a compound in flame retardants and as hardener for lead. There is still a lot of discussion on the genotoxicity of antimony compounds. Both trivalent and pentavalent compounds are generally negative in non-mammalian genotoxicity assays,...
The ToxTracker reporter assay detects indirect genotoxicity by high levels of oxidative stress
2018 — ToxTracker is a mammalian stem cell-based reporter assay that detects activation of specific cellular signaling pathways upon exposure to compounds. During the validation of this assay, we found that various compounds that give misleading positive results in the conventional in vitro genotoxicity assays did not activate the DNA damage reporters but did induce high levels of oxidative stress or protein damage in ToxTracker.
Tags: Genotoxicity,ToxTracker AO
2018 — ToxTracker is a mammalian stem cell-based reporter assay that detects activation of specific cellular signaling pathways upon exposure to compounds. During the validation of this assay, we found that various compounds that give misleading positive results in the conventional in vitro genotoxicity assays...
Validation of the ToxTracker reporter assay for the genetic toxicology assessment of petroleum products
2018 — The ToxTracker reporter cell lines were exposed to 18 DMSO extracts of petroleum substances obtained from various Concawe member companies. The differential induction of the GFP reporters as well as cytotoxicity of the tested substances was determined by flow cytometry.
Tags: Genotoxicity,Petroleum products,ToxTracker
2018 — The ToxTracker reporter cell lines were exposed to 18 DMSO extracts of petroleum substances obtained from various Concawe member companies. The differential induction of the GFP reporters as well as cytotoxicity of the tested substances was determined by flow cytometry....
Interlaboratory validation of the ToxTracker genotoxicity reporter assay
2018 — To investigate the reliability and transferability of the ToxTracker assay, we performed a limited interlaboratory validation study. In total 27 compounds (24 test compounds and 3 assay controls) were tested at least three times by two independent laboratories. The within-laboratory reproducibility (WLR) for the six different ToxTracker reporters was assessed per category of reporters (DNA damage, oxidative stress, protein damage) and for all reporters together.
Tags: Genotoxicity,ToxTracker
2018 — To investigate the reliability and transferability of the ToxTracker assay, we performed a limited interlaboratory validation study. In total 27 compounds (24 test compounds and 3 assay controls) were tested at least three times by two independent laboratories. The within-laboratory reproducibility (WLR) for...
Use of multiparametric mode of action approaches for genetic toxicity assessment: ToxTracker reporter cell lines versus gene analysis
2017 — In support of the development of a predictive, animal-free genetic toxicology approach, we investigated several different methodologies with varying degrees of complexity. The predictive capacity of these methods was compared by testing 22 chemicals that represent a mix of DNA reactive chemicals, chemicals with known, mixed or unknown modes of action, and chemicals with well-defined in vivo/vitro genotoxicity outcomes.
Tags: Genotoxicity,ToxTracker
2017 — In support of the development of a predictive, animal-free genetic toxicology approach, we investigated several different methodologies with varying degrees of complexity. The predictive capacity of these methods was compared by testing 22 chemicals that represent a mix of DNA reactive chemicals, chemicals...
Application of the ToxTracker reporter assay in a mode-of-action approach for genetic toxicology assessment
2017 — In an extensive validation study using 300 reference chemicals and >200 proprietary compounds, ToxTracker classified the genotoxic compounds with a sensitivity of 94% and specificity of 95%. By assessing the differential induction of the two DNA damage reporters, ToxTracker was able to discriminate between a mutagenic and clastogenic mechanism of genotoxicity.
Tags: Genotoxicity,ToxTracker
2017 — In an extensive validation study using 300 reference chemicals and >200 proprietary compounds, ToxTracker classified the genotoxic compounds with a sensitivity of 94% and specificity of 95%. By assessing the differential induction of the two DNA damage reporters, ToxTracker was able to discriminate...
Mechanism-based genotoxicity screening of nanomaterials using the ToxTracker panel of reporter cell lines
2017 — ToxTracker is a mammalian stem cell-based reporter assay that detects activation of specific cellular signalling pathways upon exposure to unknown compounds. ToxTracker contains six different GFP-tagged reporters that allow discrimination between induction of DNA damage, oxidative stress and protein damage in a single test.
Tags: Genotoxicity,Metals,Nanoparticles,ToxTracker
2017 — ToxTracker is a mammalian stem cell-based reporter assay that detects activation of specific cellular signalling pathways upon exposure to unknown compounds. ToxTracker contains six different GFP-tagged reporters that allow discrimination between induction of DNA damage, oxidative stress and protein damage in a single...
The ToxTracker reporter system is a mechanistic genotoxicity assay that discriminates clastogenic from aneugenic compounds
2016 — With the increasing production of new chemicals for a wide range of applications in health care, food and cosmetics, there is an urgent need for rapid and reliable toxicity assessment. For reliable prediction of human health hazards, in vitro test systems should ideally not only identify adverse properties of chemicals, but also provide insight into the type of cellular damage inflicted by novel compounds.
Tags: Genotoxicity,Pharmaceuticals,ToxTracker
2016 — With the increasing production of new chemicals for a wide range of applications in health care, food and cosmetics, there is an urgent need for rapid and reliable toxicity assessment. For reliable prediction of human health hazards, in vitro test systems should ideally...
Mechanistic toxicity profiling of chemicals and nanomaterials by combining the ToxTracker genotoxicity assay and MiToxView mitochondrial toxicity assay
2015 — The ToxTracker assay uses a panel of mouse embryonic stem (mES) cells that contain different GFP reporter genes for induction of DNA damage, oxidative stress, protein damage and general cytotoxicity. Extensive validation using different reference compound libraries as well as a collection of nanomaterials showed that the integrative approach of the ToxTracker assay is a powerful tool for in vitro carcinogenic hazard identification.
Tags: Genotoxicity,ToxTracker
2015 — The ToxTracker assay uses a panel of mouse embryonic stem (mES) cells that contain different GFP reporter genes for induction of DNA damage, oxidative stress, protein damage and general cytotoxicity. Extensive validation using different reference compound libraries as well as a collection of...
