ToxProfiler Webinar: Validation & applications of the ToxProfiler assay for mechanistic toxicity profiling
On the 14th of October, we hosted a webinar on the validation and applications of ToxProfiler, a quantitative high throughput screening platform with a single-cell resolution. The webinar is now available through the link below. During the webinar, our guest speaker, Dr. Romain Grall, also shared about his experience in the application of ToxProfiler for testing cosmetics ingredients.
ToxProfiler is a unique New Approach Method (NAM) that can be applied to accurately quantify the cellular stress responses induced by chemicals. The assay can be applied to reveal the toxicological mode-of-action (MoA) of novel medicines, (agro)chemicals, cosmetics and food ingredients. ToxProfiler consists of 7 genetically engineered HepG2 cell lines. Each cell line contains a specific fluorescent reporter that is associated with a specific cellular stress response pathway. All fluorescent reporters are expressed at physiological levels and reflect the endogenous cellular stress responses. Automated live-cell confocal microscopy and image segmentation pipelines are applied to generate a toxicological fingerprint for each compound representing the cellular stress responses that are activated upon exposure. ToxProfiler is particularly applicable for early chemical safety testing by providing insight into the toxic MoA of compounds, as well as read-across, adverse outcome pathway (AOP) and weight-of-evidence (WoE) approaches.