ToxProfiler Webinar: a quantitative high throughput screening platform with a single-cell resolution
On the 8th of April, we hosted a webinar introducing ToxProfiler, a quantitative high throughput screening platform with a single-cell resolution. The webinar is now also available through the link below. During the webinar, it was demonstrated how ToxProfiler can generate a toxicological fingerprint for each compound by identifying the cellular stress responses that are activated upon exposure to a compound. Next to this, example applications of ToxProfiler were also shared.
ToxProfiler is a unique New Approach Method (NAM) that can be applied to accurately quantify the cellular stress responses induced by chemicals. The assay can be applied to reveal the toxicological mode-of-action (MoA) of novel medicines, (agro)chemicals, cosmetics and food ingredients. ToxProfiler consists of 7 genetically engineered HepG2 cell lines. Each cell line contains a specific fluorescent reporter that is associated with a specific cellular stress response pathway. All fluorescent reporters are expressed at physiological levels and reflect the endogenous cellular stress responses. Automated live-cell confocal microscopy and image segmentation pipelines are applied to generate a toxicological fingerprint for each compound representing the cellular stress responses that are activated upon exposure. ToxProfiler is particularly applicable for early chemical safety testing by providing insight into the toxic MoA of compounds, as well as read-across, adverse outcome pathway (AOP) and weight-of-evidence (WoE) approaches.